Detection Of Extended-Spectrum Β-Lactamase (ESBL) Production in Clinical Isolates of Escherichia Coli Recovered from Patients in Tertiary Care Hospital of Gujarat

Authors

  • Vipul M Khakhkhar Gujarat Adani Institute of Medical Science (GAIMS),Bhuj, India
  • Pragnesh J Bhuva SMIMER, Surat
  • Shashwati P Bhuva SMIMER, Surat
  • Chirag P Patel Pramukhswami Medical College, Karamsad, Anand
  • Jaydev M Pandya CU Shah Medical College, Surendranagar
  • Meera S Cholera Gujarat Ayurved University, Jamnagar

Keywords:

ESBL, multi-drug resistant, MIC reduction test

Abstract

Background: The presence of Extended-Spectrum β-lactamase (ESBL) producing organisms significantly affects the course and outcome of an infection and poses a challenge to infection management worldwide. Hence, the knowledge about their prevalence is important to guide towards appropriate antibiotic treatment.

Aims & Objective: The purpose of this study is to determine the prevalence and susceptibility of ESBL in Escherichia Coli isolated from different clinical samples.

Methods: A total of 100 isolates of E. Coli were collected over a period of six months. Antimicrobial susceptibility testing was determined by commonly used antibiotics using Kirby-Bauer’s disc diffusion method. ESBL detection was done by the screening method and then confirmed by the phenotypic confirmatory test with combination disc and the minimum inhibitory concentration (MIC) reduction test as recommended by the (CLSI).

Results: Out of 100 E. coli isolates, 69 were positive by screening test and 58 were potential ESBL producers by combination disc and MIC reduction test. Multidrug resistance was significantly (p < 0.01) higher in ESBL positive isolates.

Conclusion: Knowledge of the prevalence of ESBL and resistance pattern of bacterial isolates in a geographical area will help the clinicians to formulate the guidelines for antibiotic therapy.

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Published

2012-12-31

How to Cite

1.
Khakhkhar VM, Bhuva PJ, Bhuva SP, Patel CP, Pandya JM, Cholera MS. Detection Of Extended-Spectrum Β-Lactamase (ESBL) Production in Clinical Isolates of Escherichia Coli Recovered from Patients in Tertiary Care Hospital of Gujarat. Natl J Community Med [Internet]. 2012 Dec. 31 [cited 2024 May 3];3(04):647-51. Available from: https://www.njcmindia.com/index.php/file/article/view/1786

Issue

Vol. 3 No. 04 (2012)

Section

Original Research Articles

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